Product Info Summary
SKU: | M00949-1 |
---|---|
Size: | 100ug |
Reactive Species: | Chicken, Drosophila, Human, Mouse, Rat, Yeast, Amphibians, Bacteria, Fish, Brine shrimp |
Host: | Mouse |
Application: | IP, IF, IHC, ICC, WB, Antibody Microarray |
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Product info
Product Name
Anti-HSP70 Monoclonal Antibody
View all HSP70/HSPA1A Antibodies
SKU/Catalog Number
M00949-1
Size
100ug
Form
liquid
Description
Boster Bio Anti-HSP70 Monoclonal Antibody catalog # M00949-1. Tested in IP, IF, WB applications. This antibody reacts with Human, Mouse, Rat.
Storage & Handling
Store at -20°C for one year. Avoid repeated freeze-thaw cycles.
Cite This Product
Anti-HSP70 Monoclonal Antibody (Boster Biological Technology, Pleasanton CA, USA, Catalog # M00949-1)
Host
Mouse
Contents
PBS pH7.2, 50% glycerol, 0.09% sodium azide
Clonality
Monoclonal
Clone Number
3A3
Isotype
IgG1
Immunogen
Human recombinant HSP70 overexpressed in E.coli
*Blocking peptide can be purchased. Costs vary based on immunogen length. Contact us for pricing.
Cross-reactivity
Detects ~70kDa. May detect HSP70, HSC70, p75 and HSP72.
Reactive Species
M00949-1 is reactive to HSPA1A in Chicken, Drosophila, Human, Mouse, Rat, Yeast, Amphibians, Bacteria, Fish, Brine shrimp
Applications
M00949-1 is guaranteed for IP, IF, IHC, ICC, WB, Antibody Microarray Boster Guarantee
Observed Molecular Weight
42 kDa
Calculated molecular weight
70.052kDa
Background of HSP70/HSPA1A
HSP70 genes encode abundant heat-inducible 70-kDa HSPs (HSP70s). In most eukaryotes HSP70 genes exist as part of a multigene family. They are found in most cellular compartments of eukaryotes including nuclei, mitochondria, chloroplasts, the endoplasmic reticulum and the cytosol, as well as in bacteria. The genes show a high degree of conservation, having at least 50% identity (1). The N-terminal two thirds of HSP70s are more conserved than the C-terminal third. HSP70 binds ATP with high affinity and possesses a weak ATPase activity which can be stimulated by binding to unfolded proteins and synthetic peptides (2). When HSC70 (constitutively expressed) present in mammalian cells was truncated, ATP binding activity was found to reside in an N-terminal fragment of 44 kDa which lacked peptide binding capacity. Polypeptide binding ability therefore resided within the C-terminal half (3). The structure of this ATP binding domain displays multiple features of nucleotide binding proteins (4). All HSP70s, regardless of location, bind proteins, particularly unfolded ones. The molecular chaperones of the HSP70 family recognize and bind to nascent polypeptide chains as well as partially folded intermediates of proteins preventing their aggregation and misfolding. The binding of ATP triggers a critical conformational change leading to the release of the bound substrate protein (5). The universal ability of HSP70s to undergo cycles of binding to and release from hydrophobic stretches of partially unfolded proteins determines their role in a great variety of vital intracellular functions such as protein synthesis, protein folding and oligomerization and protein transport. For more information visit our HSP70 Scientific Resource Guide at http://www.HSP70.com.
Antibody Validation
Boster validates all antibodies on WB, IHC, ICC, Immunofluorescence, and ELISA with known positive control and negative samples to ensure specificity and high affinity, including thorough antibody incubations.
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Assay dilution & Images
Assay Dilutions Recommendation
The recommendations below provide a starting point for assay optimization. The actual working concentration varies and should be decided by the user.
WB (1:5000), ICC/IF (1:500), IP (2µg); optimal dilutions for assays should be determined by the user.
Validation Images & Assay Conditions
Click image to see more details
Figure 2. Western blot analysis of HSPA1A using anti-HSPA1A antibody (M00949-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HSPA1A antigen affinity purified polyclonal antibody (Catalog # M00949-1) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1021) with Tanon 5200 system. A specific band was detected for HSPA1A.
Click image to see more details
Figure 3. Western blot analysis of HSPA1A using anti-HSPA1A antibody (M00949-1).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HSPA1A antigen affinity purified polyclonal antibody (Catalog # M00949-1) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # SA1021) with Tanon 5200 system. A specific band was detected for HSPA1A.
Click image to see more details
Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-HSP70 Monoclonal Antibody, Clone 3A3 (M00949-1) . Tissue: Cervical Cancer cell line (HeLa) . Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-HSP70 Monoclonal Antibody (M00949-1) at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: DAPI (blue) nuclear stain at 1:5000 for 5 min RT. Localization: Cytoplasm. Magnification: 40X.
Protein Target Info & Infographic
Gene/Protein Information For HSPA1A (Source: Uniprot.org, NCBI)
Gene Name
HSPA1A
Full Name
Heat shock 70 kDa protein 1A
Weight
70.052kDa
Superfamily
heat shock protein 70 family
Alternative Names
dnaK-type molecular chaperone HSP70-1; FLJ54303; FLJ54370; FLJ54392; FLJ54408; FLJ75127; Heat shock 70 kDa protein 1/2; heat shock 70 kDa protein 1A/1B; heat shock 70kD protein 1A; heat shock 70kDa protein 1A; heat shock-induced protein; HSP70; HSP70.1/HSP70.2; HSP70-1; HSP70-1/HSP70-2; HSP70-1A; HSP70I; HSP72; HSPA1; HSPA1A; HSPA1B HSPA1A HEL-S-103, HSP70-1, HSP70-1A, HSP70-2, HSP70.1, HSP70.2, HSP70I, HSP72, HSPA1 heat shock protein family A (Hsp70) member 1A heat shock 70 kDa protein 1A|HSP70-1/HSP70-2|HSP70.1/HSP70.2|Heat shock 70 kDa protein 1B|Heat shock 70 kDa protein 2|dnaK-type molecular chaperone HSP70-1|epididymis secretory protein Li 103|epididymis secretory sperm binding protein|heat shock 70 kDa protein 1|heat shock 70 kDa protein 1/2|heat shock 70 kDa protein 1A/1B|heat shock 70kD protein 1A|heat shock 70kDa protein 1A|heat shock-induced protein
*If product is indicated to react with multiple species, protein info is based on the gene entry specified above in "Species".For more info on HSPA1A, check out the HSPA1A Infographic
We have 30,000+ of these available, one for each gene! Check them out.
In this infographic, you will see the following information for HSPA1A: database IDs, superfamily, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post-translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact [email protected].
Specific Publications For Anti-HSP70 Monoclonal Antibody (M00949-1)
Hello CJ!
M00949-1 has been cited in 5 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Effects of isoliensinine on angiotensin II-induced proliferation of porcine coronary arterial smooth muscle cells
Wang,D.,Ripley-Gonzalez,J.W.& Hu,Y.Aerobic Physical Training Protects the Rat Brain Against Exercise-Heat Related Oxidative Damage through the Increased Expression of HSP70.Neurophysiology 51,66–71(2019).https://doi.org/10.1007/s11062-019-09794-9
Species: Rat
M00949-1 usage in article: APP:WB, SAMPLE:BRAIN TISSUE, DILUTION:1:400
Down-modulation of heat shock protein 70 and up-modulation of Caspase-3 during schisandrin B-induced apoptosis in human hepatoma SMMC-7721 cells
Effects of intracerebral hemorrhage and subsequent minimally invasive hematoma aspiration on expression of apoptosisrelated genes in rats.
Yi Sx, Peng Y, Chang Xr, Peng N, Yan J, Lin Yp. World J Gastroenterol. 2007 Apr 21;13(15):2174-8. Effect Of Pre-Moxibustion On Apoptosis And Proliferation Of Gastric Mucosa Cells.
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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15 Customer Q&As for Anti-HSP70 Monoclonal Antibody
Question
We are currently using anti-HSP70 Monoclonal antibody M00949-1 for rat tissue, and we are well pleased with the WB results. The species of reactivity given in the datasheet says human, mouse, rat. Is it likely that the antibody can work on zebrafish tissues as well?
Verified Customer
Verified customer
Asked: 2020-02-18
Answer
The anti-HSP70 Monoclonal antibody (M00949-1) has not been validated for cross reactivity specifically with zebrafish tissues, though there is a good chance of cross reactivity. We have an innovator award program that if you test this antibody and show it works in zebrafish you can get your next antibody for free. Please contact me if I can help you with anything.
Boster Scientific Support
Answered: 2020-02-18
Question
Would anti-HSP70 Monoclonal antibody M00949-1 work for IP with embryonic kidney?
Verified Customer
Verified customer
Asked: 2020-01-15
Answer
According to the expression profile of embryonic kidney, HSPA1A is highly expressed in embryonic kidney. So, it is likely that anti-HSP70 Monoclonal antibody M00949-1 will work for IP with embryonic kidney.
Boster Scientific Support
Answered: 2020-01-15
Question
Is a blocking peptide available for product anti-HSP70 Monoclonal antibody (M00949-1)?
Verified Customer
Verified customer
Asked: 2019-12-25
Answer
We do provide the blocking peptide for product anti-HSP70 Monoclonal antibody (M00949-1). If you would like to place an order for it please contact [email protected] and make a special request.
Boster Scientific Support
Answered: 2019-12-25
Question
See below the WB image, lot number and protocol we used for embryonic kidney using anti-HSP70 Monoclonal antibody M00949-1. Please let me know if you require anything else.
Verified Customer
Verified customer
Asked: 2019-11-22
Answer
Thank you very much for the data. Our lab team are working to resolve this as quickly as possible, and we appreciate your patience and understanding! You have provided everything we needed. Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2019-11-22
Question
I am interested in to test anti-HSP70 Monoclonal antibody M00949-1 on mouse embryonic kidney for research purposes, then I may be interested in using anti-HSP70 Monoclonal antibody M00949-1 for diagnostic purposes as well. Is the antibody suitable for diagnostic purposes?
Verified Customer
Verified customer
Asked: 2019-08-08
Answer
The products we sell, including anti-HSP70 Monoclonal antibody M00949-1, are only intended for research use. They would not be suitable for use in diagnostic work. If you have the means to develop a product into diagnostic use, and are interested in collaborating with us and develop our product into an IVD product, please contact us for more discussions.
Boster Scientific Support
Answered: 2019-08-08
Question
I was wanting to use your anti-HSP70 Monoclonal antibody for IP for mouse embryonic kidney on frozen tissues, but I want to know if it has been tested for this particular application. Has this antibody been tested and is this antibody a good choice for mouse embryonic kidney identification?
Verified Customer
Verified customer
Asked: 2019-06-05
Answer
You can see on the product datasheet, M00949-1 anti-HSP70 Monoclonal antibody has been validated for IP, IF, WB on human, mouse, rat tissues. We have an innovator award program that if you test this antibody and show it works in mouse embryonic kidney in IHC-frozen, you can get your next antibody for free.
Boster Scientific Support
Answered: 2019-06-05
Question
I see that the anti-HSP70 Monoclonal antibody M00949-1 works with IP, what is the protocol used to produce the result images on the product page?
Verified Customer
Verified customer
Asked: 2019-05-20
Answer
You can find protocols for IP on the "support/technical resources" section of our navigation menu. If you have any further questions, please send an email to [email protected]
Boster Scientific Support
Answered: 2019-05-20
Question
We have tried in the past anti-HSP70 Monoclonal antibody for WB on uterus a few years ago. I am using human, and We intend to use the antibody for IP next. I am looking for examining uterus as well as colon carcinoma in our next experiment. Could give a recommendation on which antibody would work the best for IP?
F. Singh
Verified customer
Asked: 2019-04-12
Answer
I have checked the website and datasheets of our anti-HSP70 Monoclonal antibody and it seems that M00949-1 has been tested on human in both WB and IP. Thus M00949-1 should work for your application. Our Boster satisfaction guarantee will cover this product for IP in human even if the specific tissue type has not been validated. We do have a comprehensive range of products for IP detection and you can check out our website bosterbio.com to find out more information about them.
Boster Scientific Support
Answered: 2019-04-12
Question
Does M00949-1 anti-HSP70 Monoclonal antibody work on parafin embedded sections? If so, which fixation method do you recommend we use (PFA, paraformaldehyde, other)?
Verified Customer
Verified customer
Asked: 2018-09-28
Answer
As indicated on the product datasheet, M00949-1 anti-HSP70 Monoclonal antibody as been validated on IP. It is best to use PFA for fixation because it has better tissue penetration ability. PFA needs to be prepared fresh before use. Long term stored PFA turns into formalin, as the PFA molecules congregate and become formalin.
Boster Scientific Support
Answered: 2018-09-28
Question
I have a question about product M00949-1, anti-HSP70 Monoclonal antibody. I was wondering if it would be possible to conjugate this antibody with biotin. I would need it to be without BSA or sodium azide. I am planning on using a buffer exchange of sodium azide with PBS only. Would there be problems for me to conjugate the antibody and store it in -20 degrees in small aliquots?
Verified Customer
Verified customer
Asked: 2018-08-03
Answer
We do not recommend storing this antibody with PBS buffer only in -20 degrees. If you want to store it in -20 degrees it is best to add some cryoprotectant like glycerol. If you want carrier free M00949-1 anti-HSP70 Monoclonal antibody, we can provide it to you in a special formula with trehalose and/or glycerol. These molecules will not interfere with conjugation chemistry and provide a good level of protection for the antibody from degradation. Please be sure to specify this in your purchase order.
Boster Scientific Support
Answered: 2018-08-03
Question
Our lab were content with the WB result of your anti-HSP70 Monoclonal antibody. However we have been able to see positive staining in pancreas cytoplasm. using this antibody. Is that expected? Could you tell me where is HSPA1A supposed to be expressed?
Verified Customer
Verified customer
Asked: 2018-04-27
Answer
Based on literature, pancreas does express HSPA1A. Generally HSPA1A expresses in cytoplasm. Regarding which tissues have HSPA1A expression, here are a few articles citing expression in various tissues:
Brain, Cajal-Retzius cell, and Fetal brain cortex, Pubmed ID: 23349634
Brain, Muscle, Pancreas, PNS, and Skin, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 17081983, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Colon carcinoma, Pubmed ID: 24129315
Liver, Pubmed ID: 24275569
Uterus, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2018-04-27
Question
We have been able to see staining in rat endothelial cell. Do you have any suggestions? Is anti-HSP70 Monoclonal antibody supposed to stain endothelial cell positively?
Verified Customer
Verified customer
Asked: 2017-12-27
Answer
Based on literature endothelial cell does express HSPA1A. Based on Uniprot.org, HSPA1A is expressed in endothelial cell, uterus, brain, muscle, pancreas, pns skin, embryonic kidney, brain, cajal-retzius cell fetal brain cortex, cervix carcinoma, cervix carcinoma erythroleukemia, liver, colon carcinoma, among other tissues. Regarding which tissues have HSPA1A expression, here are a few articles citing expression in various tissues:
Brain, Cajal-Retzius cell, and Fetal brain cortex, Pubmed ID: 23349634
Brain, Muscle, Pancreas, PNS, and Skin, Pubmed ID: 15489334
Cervix carcinoma, Pubmed ID: 17081983, 18691976, 20068231
Cervix carcinoma, and Erythroleukemia, Pubmed ID: 23186163
Colon carcinoma, Pubmed ID: 24129315
Liver, Pubmed ID: 24275569
Uterus, Pubmed ID: 14702039
Boster Scientific Support
Answered: 2017-12-27
Question
Is this M00949-1 anti-HSP70 Monoclonal antibody reactive to the isotypes of HSPA1A?
T. Miller
Verified customer
Asked: 2016-06-22
Answer
The immunogen of M00949-1 anti-HSP70 Monoclonal antibody is Human recombinant HSP70 overexpressed in E.coli. Could you tell me which isotype you are interested in so I can help see if the immunogen is part of this isotype?
Boster Scientific Support
Answered: 2016-06-22
Question
We appreciate helping with my inquiry over the phone. Here are the WB image, lot number and protocol we used for embryonic kidney using anti-HSP70 Monoclonal antibody M00949-1. Let me know if you need anything else.
C. Huang
Verified customer
Asked: 2013-10-03
Answer
We appreciate the data. You have provided everything we needed. Our lab team are working to resolve your inquiry as quickly as possible, and we appreciate your patience and understanding! Please let me know if there is anything you need in the meantime.
Boster Scientific Support
Answered: 2013-10-03
Question
Do you have a BSA free version of anti-HSP70 Monoclonal antibody M00949-1 available?
R. Yang
Verified customer
Asked: 2013-01-09
Answer
We appreciate your recent telephone inquiry. I can confirm that some lots of this anti-HSP70 Monoclonal antibody M00949-1 are BSA free. For now, these lots are available and we can make a BSA free formula for you free of charge. It will take 3 extra days to prepare. If you require this antibody BSA free again in future, please do not hesitate to contact me and I will be pleased to check which lots we have in stock that are BSA free.
Boster Scientific Support
Answered: 2013-01-09